| 朱丹萍,罗敬铭,蔡鑫君.超高效液相色谱-串联质谱法测定人血清中美罗培南浓度的研究[J].浙江中西医结合杂志,2023,33(11): |
| 超高效液相色谱-串联质谱法测定人血清中美罗培南浓度的研究 |
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| 投稿时间：2023-04-28 修订日期：2023-10-25 |
| DOI： |
| 中文关键词: 美罗培南，治疗药物监测，超高效液相色谱串联质谱法 |
| 英文关键词:meropenem, therapeutic drug monitoring, ultra performance liquid chromatography tandem mass spectrometry |
| 基金项目: |
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| 摘要点击次数: 435 |
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| 中文摘要: |
| 目的：建立一种快速准确的超高效液相色谱串联质谱法，测定人血清中美罗培南的浓度。方法：人血清样品采用甲醇蛋白质沉淀法处理后，加入内标。采用ZORBAX PRHD SB-Aq色谱柱，采用0.1%甲酸水和甲醇为流动相梯度洗脱。质谱扫描模式为正离子模式，监测的离子为美罗培南和内标美罗培南-D6。随后考察了该检测方法的选择性，准确度，精密度，回收率，基质效应，残留效应等。结果，经过验证，该方法的选择性强。美罗培南的回归方程为y=0.136997*x-0.00423401（R2=0.997），在0.5~100 μg/mL的浓度范围内线性良好，定量下限为0.5 μg/mL。日内和日间准确度和精密度都在限定范围内。基本没有基质效应和残留效应。结论：该方法选择性好，灵敏度高，操作简单，能快速准确的完成人血清中的美罗培南浓度检测。 |
| 英文摘要: |
| Objective: To establish a rapid and accurate ultra performance liquid chromatography tandem mass spectrometry method to determine the concentration of meropenem in human serum. Methods: Human serum samples were processed by methanolic protein precipitation method and added to the internal standard. A ZORBAX PRHD SB-Aq column was used with a gradient elution of 0.1% formic acid water and methanol as mobile phases. The mass spectrometry scan mode was positive ion mode, and the monitored ions were meropenem and the internal standard meropenem-D6. The selectivity, accuracy, precision, recovery, matrix effect, and residual effect of the assay were subsequently investigated. As a result, the method was validated to be highly selective. The regression equation for meropenem was y = 0.136997*x-0.00423401 (R2 = 0.997) with good linearity in the concentration range of 0.5 to 100 μg/mL and a lower limit of quantification of 0.5 μg/mL. intra-day and inter-day accuracy and precision were within limits. There was essentially no matrix effect or carry over. Conclusion: The method is selective, sensitive and simple to operate, and can rapidly and accurately complete the determination of meropenem concentration in human serum. |
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