| 刘翔,汤绚丽,余瑾.尿沉渣中钙信号相关mRNA作为IgA肾病足细胞损伤无创性指标的研究[J].浙江中西医结合杂志,2024,34(1): |
| 尿沉渣中钙信号相关mRNA作为IgA肾病足细胞损伤无创性指标的研究 |
| Study on calcium signal related mRNA in urinary sediment as noninvasive indicator of podocytes injury in IgA nephropathy |
| 投稿时间：2023-05-08 修订日期：2023-12-08 |
| DOI： |
| 中文关键词: IgA肾病 足细胞损伤 TRPC6 mRNA Calpain mRNA 无创性指标 |
| 英文关键词:IgA nephropathy Urinary podocyte injury TRPC6 mRNA Calpain mRNA Non-invasive index |
| 基金项目:浙江省医药卫生科技计划(编号:2023RC245) |
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| 中文摘要: |
| 目的:探讨尿沉渣中与钙信号通路相关的TRPC6及Calpain mRNA及骨架蛋白Talin-1 mRNA、synaptopodin mRNA是否能作为评估IgA肾病 (IgA nephropathy,IgAN)足细胞损伤的无创性监测指标。方法:选取36例IgAN患者为研究对象,收集患者的临床资料、肾活检病理和尿液标本,同时收集8例健康体检人群的尿液作为对照组。分别提取尿沉渣中的钙信号相关蛋白TRPC6以及Calpain、骨架蛋白Talin-1、synaptopodin mRNA,比较病例组与健康对照组的mRNA差异,明确尿TRPC6 mRNA与肾组织TRPC6表达间的关系。并进行尿液mRNA水平与临床病理指标相关性分析,及治疗后的动态监测。结果:与健康对照组相比,IgAN组尿中Calpain-1 mRNA 较健康组有统计学差异(P<0. 05),TRPC6 mRNA有明显升高趋势；尿TRPC6 mRNA水平能反应肾组织中的TRPC6沉积；尿TRPC6 mRNA与蛋白尿分级水平呈现正相关(r=0.407,P=0.049),与足细胞融合的程度有正相关(r=0.452,P=0.027)。尿Calpain mRNA与蛋白尿分级水平呈现正相关(r=0.493,P=0.014),与MAP也呈正相关(r=0.469,P=0.021)；伴有尿足细胞阳性的患者其尿TRPC6 mRNA水平及Calpain-1 mRNA 表达高于足细胞阴性组(P<0.05),治疗2月后,伴随24小时尿蛋白定量减少及MAP下降,尿TRPC6 mRNA及Calpain-1 mRNA水平下降,具有统计学差异(P=0.042,P=0.009)。 |
| 英文摘要: |
| Objective To assess whether TRPC6, Calpain mRNA, Talin-1 mRNA and synaptopodin mRNA related to calcium signaling pathway in urinary sediment can be used as noninvasive monitoring indicators to evaluate IgA nephropathy (IgAN) podocytes injury. Methods 36 patients with IgAN were selected as subjects, clinical data, renal biopsy pathology and urine samples were collected, and urine samples of 8 healthy people were collected as control group. Calcium signal-related protein TRPC6, Calpain, skeleton proteins Talin-1, and synaptopodin mRNA were extracted from urinary sediment, respectively. The different of mRNA between case group and healthy control group were compared, and the relationship between urinary TRPC6 mRNA and TRPC6 expression in renal tissue was determined. The correlation between urine mRNA level and clinicopathological index was analyzed, and the dynamic monitoring was performed after treatment. Results Compared with healthy control group, Calpain-1 mRNA in urine of IgAN group was significantly different from that of healthy group (P< 0.05), and TRPC6 mRNA showed a significant increase trend. Urinary TRPC6 mRNA level can reflect TRPC6 deposition in renal tissue. Urinary TRPC6 mRNA was positively correlated with albuminuria grading level (r=0.407, p=0.049), and the degree of podocytes fusion (r=0.452, p=0.027). Urinary Calpain mRNA was positively correlated with albuminuria grading level (r=0.493, p=0.014), and MAP was also positively correlated (r=0.469, p=0.021). Urine TRPC6 mRNA level and Calpain-1 mRNA expression in podocyte-positive patients were higher than those in podocyte-negative group (p<0.05). After 2 months of treatment, the levels of urinary TRPC6 mRNA and Calpain-1 mRNA were decreased with the decrease of 24-hour urinary protein quantity and MAP, with statistical difference (p=0.042, p=0.009). Conclusion TRPC6 mRNA and Calpain mRNA may be used as noninvasive monitoring indicators to evaluate podocyte injury and efficacy in IgA nephropathy. |
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