| 李杰,周邦瑜,马彦博,李绍波.基于网络药理学探究补阳五汤治疗脊髓损伤的成分和机制[J].浙江中西医结合杂志,2023,33(11): |
| 基于网络药理学探究补阳五汤治疗脊髓损伤的成分和机制 |
| Component and mechanism of buyangwu decoction in the treatment of spinal cord injury based on network pharmacologyLi Jie1 Zhou Bang Yu1 Ma Yan Bo1 Li Shao Bo2 |
| 投稿时间：2023-05-25 修订日期：2023-07-13 |
| DOI： |
| 中文关键词: 补阳还五汤 脊髓损伤 网络药理学 槲皮素 山奈酚 |
| 英文关键词:Buyang Huanwu Decoction Spinal cord injury Network pharmacology Quercetin Kaempferol |
| 基金项目:云南省科技厅高校联合项目-面上项目（2019FH001-015） 云南省大理大学第一附属医院学科队伍建设重点项目（DFYZD 2002-02）云南省教育厅科学研究基金项目（2023Y0972） |
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| 中文摘要: |
| 目的 探究补阳五汤治疗脊髓损伤的成分和机制。方法 通过TCMSP数据库和文献查找相结合的方法筛选补阳还五汤的成分和相应靶点,构建 “中药-成分-靶点” 网络图。以“spine core injure”为检索词在Genecards数据库中检索髓损伤的治疗靶点,与药物靶点取交集构建Venn图。导入交集靶点到STRING中构建蛋白质-蛋白质网络,用Cytoscape软件筛选补阳还五汤治疗脊髓损伤的关键靶点。导入关键靶点到DAVID网站,进行GO和KEGG分析,并进行可视化。利用分子对接技术来模拟主要成分与核心靶点的结合情况。结果 筛选到63个补阳还五汤成分和106个交集靶点。其中槲皮素、山柰酚、β-谷甾醇是主要成分,P53、AKT1、MAPK1、RELA是核心靶点,MAPK、PI3K-Akt、FoxO、Toll是主要信号通路。分子对接显示主要成分与核心靶点间均能稳定结合。结论 补阳还五汤通过多成分、多靶点、多通道来实现脊髓损伤的治疗。 |
| 英文摘要: |
| Objective To explore the components and mechanism of buyangwu decoction in the treatment of spinal cord injury. Methods The components and corresponding targets of Buyang Huanwu Decoction were screened by the combination of TCMSP database and literature search, and the network diagram of "traditional Chinese medicine–components–targets" was constructed. The therapeutic targets of pulp injury were retrieved in the Genecards database using "spine core injure" as the search term, and the Venn map was constructed by intersecting with the drug targets. Protein-protein network was constructed by importing the intersection target points into STRING, and the key targets of Buyang Huanwu Decoction in the treatment of SCI were screened using Cytoscape software. Import key targets to DAVID"s website for GO and KEGG analysis and visualization. Molecular docking technology was used to simulate the binding of main components to core targets. Results 63 components of Buyang Huanwu Decoction and 106 intersecting targets were screened out. Quercetin, kaempferol and β-sitosterol were the main components, P53, AKT1, MAPK1 and RELA were the core targets, and MAPK, PI3K-Akt, FoxO and Toll were the main signaling pathways. Molecular docking showed stable binding between the main components and the core targets. Conclusion Buyang Huanwu Decoction realizes the treatment of SCI through multi-component, multi-target and multi-channel. |
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