| 金哲柱,金永军,余立军,蒋三亚,许文彬,余育俊.生漆提取物联合OGFr对人肝癌细胞HepG2.0活性的抑制作用[J].浙江中西医结合杂志,2025,35(5): |
| 生漆提取物联合OGFr对人肝癌细胞HepG2.0活性的抑制作用 |
| Inhibitory effect of lacquer extract combined with OGFr on the activity of human liver cancer cell line HepG2.0 |
| 投稿时间：2023-06-29 修订日期：2025-01-09 |
| DOI： |
| 中文关键词: 肝癌 生漆? ONC201 OGF |
| 英文关键词:Liver cancer Lacquer ONC201 OGF |
| 基金项目: |
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| 中文摘要: |
| 目的: 研究了RLE对人肝癌细胞HepG2.0的治疗作用，并探讨了OGFr联合RLE诱导的抗肿瘤活性的可能协同作用。方法:RT-PCR法检测信号分子在人肝癌细胞(HepG2)中的表达, 并使用RLE或ONC201处理正常结肠上皮细胞NCM460，以评估RLE的影响；流式细胞术检测表达OGFr的人肝癌细胞在生漆提取物作用下发生G0/G1期阻滞，凋影响；Western blot检测凋亡相关蛋白表达。结果: RLE-OGFr 重组质粒转染人肝癌细胞，发现人肝癌细胞(HepG)中存在 OGFr 的表达，转染OGFr 重组质粒可进一步增加细胞中OGFr 表达水平，重组质粒成功转入细胞，并可上调OGFr蛋白的表达。ONC201和生漆提取物（RLE）对人肝癌细胞具有较强的抗肿瘤活性。同时，OGFr和RLE也抑制了肝癌细胞的迁移和侵袭能力。重要的是，RLE、OGF和ONC201共同处理大大增强了肝癌细胞中的抗抗肿瘤活性结论: RLE、OGF和ONC201共同处理增强了肝癌细胞中的抗抗肿瘤活性，抑制Shh高表达的人肝癌细胞生长,阻滞细胞周期于G0/G1期,并诱导肝癌细胞凋亡。 |
| 英文摘要: |
| Objective: To investigate the therapeutic effect of RLE on human liver cancer cell line HepG2.0 and explore the possible synergistic effect of OGFr combined with RLE induced anti-tumor activity. Methods: RT-PCR was used to detect the expression of Signaling molecule in human hepatoma cells (HepG2), and RLE or ONC201 was used to treat normal colon epithelial cells NCM460 to evaluate the effect of RLE; Flow cytometry was used to detect the G0/G1 phase arrest and apoptosis of human liver cancer cells expressing OGFr under the action of lacquer extract; Western blot was used to detect the expression of apoptosis related proteins. Result: The RLE-OGFr recombinant plasmid was transfected into human liver cancer cells and the expression of OGFr was found in human liver cancer cells (HepG). Transfection with the OGFr recombinant plasmid further increased the expression level of OGFr in the cells. The recombinant plasmid was successfully transfected into the cells and upregulated the expression of OGFr protein. ONC201 and Lacquer Extract (RLE) have strong anti-tumor activity against human liver cancer cells. Meanwhile, OGFr and RLE also inhibit the migration and invasion ability of liver cancer cells. Importantly, RLE, OGF and ONC201 co treatment significantly enhanced the anti-tumor activity in hepatoma cells Conclusion: RLE, OGF and ONC201 co treatment enhanced the anti-tumor activity in hepatoma cells, inhibited the growth of human hepatoma cells with high Shh expression, blocked cell cycle in G0/G1 phase, and induced apoptosis of hepatoma cells. |
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