| 俞坤强,马旭,徐日,殷冬雪,吴李秀,林如辉.电针调节转运蛋白改善MCAO大鼠脑内葡萄糖代谢和空间学习记忆的机制研究[J].浙江中西医结合杂志,2024,34(7): |
| 电针调节转运蛋白改善MCAO大鼠脑内葡萄糖代谢和空间学习记忆的机制研究 |
| Mechanism of electroacupuncture in promotion of glucose metabolism and spatial learning memory by regulating transporters in a rat model of middle cerebral artery occlusion |
| 投稿时间：2023-07-31 修订日期：2024-06-17 |
| DOI： |
| 中文关键词: 脑梗死 大脑中动脉栓塞 葡萄糖代谢 葡萄糖转运蛋白-1 葡萄糖转运蛋白-3 学习记忆 |
| 英文关键词:Cerebral infarction Middle cerebral artery embolism Glucose metabolism Glucose transporter- 1 Glucose transporter- 3 Learning and memory |
| 基金项目:浙江省自然科学基金(No. LQ20H270020) |
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| 中文摘要: |
| 目的 探讨电针对大脑中动脉闭塞（MCAO）模型大鼠脑内葡萄糖代谢和空间学习记忆的影响及相关机制。方法 40只 SD 大鼠随机数字表法分为假手术组、模型组、电针组、非穴组，每组 10 只。采用线栓塞法建立 MCAO 模型大鼠，电针组给予电针百会和神庭穴，20min每次，每天1次，治疗7d，非穴组电针非穴，余同电针组，假手术组和模型组给予同等时间的抓取和固定。观察各组大鼠神经功能评分，水迷宫空间探索实验和定向巡航实验结果，TTC检测脑梗死体积，PET-CT检测海马区葡萄糖代谢，蛋白质印迹检测转运蛋白1（GLUT1）和转运蛋白3（GLUT3）的表达水平。结果 与模型组比较，电针组大鼠第5、第7d神经功能评分显著降低[（1.553±0.511）分比（2.047±0.453）分，（1.303±0.469）比（1.958±0.260）分，均P＜0.05]；第3、4、5、6d逃避潜伏期明显缩短 [（58.653±10.545）秒比（86.183±15.861）秒、（45.163±11.758）秒比（73.889±14.853）秒、（36.480±11.267）秒比（63.234±13.873）秒，（25.413±8.725）秒比（50.768±11.567）秒，P 均＜0.05]；穿越平台次数增加[（2.930±0.949）次比（1.533±0.525）次，P＜0.05];左侧海马区葡萄糖摄取量增加[（86.745±25.127）%比（80.282±24.000）%，P＜0.05];脑组织含水量减少[（78.271±0.600）%比（84.446±1.084）%，P＜0.05]；左侧海马区GLUT1和GLUT3表达水平增加 [（73.220±10.478）%比（56.385±12.205）%、（79.176±9.824）%比（58.373±16.067）%，P 均＜0.05]。与非穴组比较，电针组大鼠第5、第7d神经功能评分显著降低[（1.553±0.511）分比（2.031±0.453）分，（1.303±0.469）分比（1.823±0.473）分，均P＜0.05]；第3、4、5、6d逃避潜伏期明显缩短 [（58.653±10.545）秒比（84.478±14.359）秒、（45.163±11.758）秒比（70.780±13.932）秒、（36.480±11.267）秒比（59.940±11.015）秒，（25.413±8.725）秒比（48.965±10.438）秒，P 均＜0.05]；穿越平台次数增加[（2.930±0.949）次比（1.625±0.504）次，P＜0.05];左侧海马区葡萄糖摄取量增加[（86.745±25.127）%比（81.866±25.674）%，P＜0.05];脑组织含水量减少[（78.271±0.600）次比（82.444±0.396）次，P＜0.05]；左侧海马区GLUT1和GLUT3表达水平增加 [（73.220±10.478）%比（58.573±8.735）%、（79.176±9.824）%比（62.099±9.981）%，P 均＜0.05]。结论 电针百会、神庭穴可能通过调节GLUT1和GULT2的表达改善脑内葡萄糖代谢水平从而改善MCAO 模型大鼠空间学习记忆能力。 |
| 英文摘要: |
| Objective To assess the effects of electroacupuncture in promotion of glucose metabolism and spatial learning memory in a rat model of the middle cerebral artery occlusion （MCAO）and then explored the underlying molecular events. Methods Forty Sprague -Dawley rats were randomly divided into sham operation, MCAO model group, electroacupuncture group（EA） and non-acupoint group（n=10 per group）. The model rats were established by thread embolization。 The EA group was given electroacupuncture at Baihui and Shenting points, once a day for 7 days. The non-acupoint group was given EA at non-acupoints, and the sham-operation group and the model group were given equal time of grasping and fixation.Neural function scores and water maze test results were observed in each group. TTC was used to detect cerebral infarction volume, PET-CT was used to detect glucose metabolism in hippocampus region, and western blot was used to detect the expression levels of transporter 1 (GLUT1) and transporter 3 (GLUT3). Results Compared with the model group , the EA group showed decrease in the neural function scores on the 5th and 7th day (1.553±0.511 vs. 2.047±0.453, 1.303±0.469 vs. 1.958±0.260; P＜0.05) and the latency of escape on the 3th，4th，5th and 6th (58.653±10.545 vs. 86.183±15.861, 45.163±11.758 vs. 73.889±14.853, 36.480±11.267 vs.63.234±13.873, 25.413±8.725 vs. 50.768±11.567; all P＜0.05) and the water content of brain tissue (78.271±0.600 vs. 84.446±1.08; P＜0.05), but decrease in the number of crossing the platform (2.930±0.949 vs. 1.533±0.525; P＜0.05) and glucose uptake in left hippocampus (86.745±25.127 vs.80.282±24.000; P＜0.05) and the expression levels in the left hippocampus of GLUT1 (73.220±10.478 vs.56.385±12.205; ; P＜0.05) and the expression levels in the left hippocampus of GLUT3 (79.176±9.824 vs. 58.373±16.067; P＜0.05). Compared with the non-acupoint group , the EA group showed decrease in the neural function scores on the 5th and 7th day (1.553±0.511 vs. 2.031±0.453, 1.303±0.469 vs. 1.823±0.473; all P＜0.05) and the latency of escape on the 3th，4th，5th and 6th (58.653±10.545 vs. 84.478±14.359, 45.163±11.758 vs. 70.780±13.932, 36.480±11.267 vs.59.940±11.015, 25.413±8.725 vs. 48.965±10.438; all P＜0.05) and the water content of brain tissue (78.271±0.600 vs. 82.444±0.396; P＜0.05), but decrease in the number of crossing the platform (2.930±0.949 vs. 1.625±0.504; P＜0.05) and glucose uptake in left hippocampus (86.745±25.127 vs.81.866±25.674; P＜0.05) and the expression levels in the left hippocampus of GLUT1 (73.220±10.478 vs.58.573±8.735; P＜0.05) and the expression levels in the left hippocampus of GLUT3 (79.176±9.824 vs. 62.099±9.981; P＜0.05). Conclusion Electroacupuncture at Baihui and Shiting points may improve the spatial learning and memory ability of MCAO rats by regulating the levels of GLUT1 and GULT2 to improve brain glucose metabolism. |
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