| 石明晴,王津,徐小雨,蓝关翠.Kiss-1/GPR54系统在PCOS大鼠卵巢颗粒细胞中的表达 及对卵泡发育障碍的作用机制[J].浙江中西医结合杂志,2024,34(11): |
| Kiss-1/GPR54系统在PCOS大鼠卵巢颗粒细胞中的表达 及对卵泡发育障碍的作用机制 |
| The expression of Kiss-1/GPR54 system in ovarian granulosa cells of PCOS rats and its mechanism of action on follicular developmental disorders |
| 投稿时间：2023-12-30 修订日期：2024-10-30 |
| DOI： |
| 中文关键词: 多囊卵巢综合征 颗粒细胞 卵泡发育 亲吻素 G蛋白偶联受体54 |
| 英文关键词:Polycystic ovary syndrome rats Granular cells Follicular development Kisspeptin G protein coupled receptor 54 |
| 基金项目:浙江省中医药科学研究基金项目（2021ZB338）；2021年浙江省卫生健康科技计划项目（2021RC147） |
|
| 摘要点击次数: 313 |
| 全文下载次数: 3 |
| 中文摘要: |
| [目的]探讨Kiss-1/GPR54系统在多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞中的表达,并分析其与PCOS之间的关联。[方法]筛选出14只动情规律的雌性SD大鼠,随机分为对照组和模型组(每组7只)。其中模型组大鼠予来曲唑-羧甲基纤维素混悬液0.1mg/(kg/d)连续灌胃21天以构建PCOS大鼠模型,收集大鼠血清、卵巢组织、卵巢颗粒细胞。然后计算大鼠卵巢系数,对大鼠卵巢组织进行苏木精-伊红染色法,采用酶联免疫吸附测定法检测血清激素水平,免疫荧光染色检测大鼠颗粒细胞中亲吻素(Kp)、G蛋白偶联受体54(GPR54)荧光强度,实时定量反转录聚合酶链式反应检测大鼠颗粒细胞中Kiss-1、GPR54基因表达。[结果]与正常对照组比较,模型组大鼠卵巢囊状扩张卵泡及闭锁卵泡增加,卵泡颗粒细胞层变薄；模型组大鼠卵巢系数增大,并且具有统计学差异(P<0.05),血清中黄体生成素、睾酮、Kp水平升高(P<0.05),卵巢颗粒细胞中Kp、GPR54荧光强度及Kiss-1、GPR54基因表达降低(P<0.05)。[结论]卵巢表达的Kiss-1/GPR54系统可能通过调节颗粒细胞而影响PCOS大鼠卵泡发育,本研究将为PCOS卵泡发育失常的发病机理的深入探索提供一定的理论依据。 |
| 英文摘要: |
| [Objective] To investigate the expression of Kiss-1/GPR54 system in earic granulosa cells of mice, which were suffering from polycystic ovary syndrome (PCOS), and to analyze the correlatiion between PCOS and KISS-1 /GPR54 system. [Method] The study selected fourteen SD mice of femina with law periods of love, which were randomized dividedly into the normal control group and model group (every group consisted of 7 rats). The model group rats were given a 0.1mg/kg/d suspension of letrozole carboxymethyl cellulose by gavage continuously for 21 days to construct a PCOS rat model. The serum, ovarian tissue, and granulosa cells of the rats were collected. Then calculate the ovarian coefficient of rats, perform hematoxylin eosin staining on the ovarian tissue of rats, use enzyme-linked immunosorbent assay to detect serum hormone levels, immunofluorescence staining to detect kisspeptin (Kp) and G protein coupled receptor 54 (GPR54) fluorescence intensity in rat granulosa cells, and real-time quantitative reverse transcription polymerase chain reaction to detect Kiss-1 in rat granulosa cells GPR54 gene expression. [Results] The model group, compared to the normal control group, represented that the saccular dilation and cleidoic follicles were augementing and the tier of granular cellula became thinner in follicles; The ovarian coefficient of the model group rats increased and showed statistical differences (P<0.05). The levels of luteinizing hormone, testosterone, and Kp in serum increased (P<0.05), while the fluorescence intensity of Kp and GPR54 in ovarian granulosa cells and the expression of Kiss-1 and GPR54 genes decreased (P<0.05). [Conclusion] The Kiss-1/GPR54 system expressed in the ovary may affect follicular development in PCOS rats by regulating granulosa cells. This study will provide a theoretical basis for further exploring the pathogenesis of follicular developmental disorders in PCOS. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
