| 陈乃君,高倩.小白菊内酯调节JAK/STAT信号通路对高糖诱导胰岛β细胞损伤的保护作用[J].浙江中西医结合杂志,2024,34(11): |
| 小白菊内酯调节JAK/STAT信号通路对高糖诱导胰岛β细胞损伤的保护作用 |
| Protective effect of parthenolide on islet β cell injury induced by high glucose by regulating JAK/STAT signal pathway |
| 投稿时间：2024-01-20 修订日期：2024-10-30 |
| DOI： |
| 中文关键词: 小白菊内酯 Janus激酶/信号转导和转录激活子信号通路 高糖 胰岛β细胞 凋亡 |
| 英文关键词:: parthenolide Janus kinase/signal transducer and activator of transcription signal pathway High glucose Islet β cells Apoptosis |
| 基金项目:浙江省医药卫生科技计划项目(2018KY838) |
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| 中文摘要: |
| 【】目的:探讨小白菊内酯(PTL)调节Janus激酶(JAK)/信号转导和转录激活子(STAT)信号通路对高糖(HG)诱导胰岛β细胞损伤的影响。方法:分别用 0、2.5、5、10、20、40μmol/L PTL处理HG诱导的小鼠胰岛β细胞MIN6细胞,通过检测细胞活力筛选出合适的处理MIN6细胞的PTL浓度；取对数生长期的MIN6细胞,分组为对照组(5.5 mmol/L葡萄糖)、HG组(33.3 mmol/L葡萄糖)、HG+低剂量PTL组(33.3 mmol/L葡萄糖+2.5μmol/L PTL)、HG+中剂量PTL组(33.3 mmol/L葡萄糖+5μmol/L PTL)、HG+高剂量PTL组(33.3 mmol/L葡萄糖+10μmol/L PTL)、HG+AG490(JAK/STAT通路抑制剂)组(33.3 mmol/L葡萄糖+40μmol/L AG490)、HG+高剂量PTL+AG490组(33.3 mmol/L葡萄糖+10μmol/L PTL+40μmol/L AG490)。CCK-8检测MIN6细胞增殖；流式细胞术检测MIN6细胞凋亡；试剂盒检测细胞上清液中白细胞介素-6 (IL-6)、肿瘤坏死因子-α(TNF-α)水平及胰岛素含量；western blot检测MIN6细胞中增殖细胞核抗原(PCNA)、BCL2相关X蛋白(Bax)、p-JAK2、p-STAT3蛋白表达。结果:选择2.5μmol/L、5μmol/L、10μmol/L PTL作为处理HG诱导的MIN6细胞的低、中、高剂量浓度。与对照组比较,HG组OD450值、胰岛素含量、PCNA、p-JAK2、p-STAT3蛋白表达降低,细胞凋亡率、IL-6、TNF-α水平、Bax蛋白表达升高(P<0.05)；与HG组比较,HG+低剂量PTL组、HG+中剂量PTL组、HG+高剂量PTL组OD450值、胰岛素含量、PCNA、p-JAK2、p-STAT3蛋白表达升高,细胞凋亡率、IL-6、TNF-α水平、Bax蛋白表达降低(P<0.05)；与HG组比较,HG+AG490组OD450值、胰岛素含量、PCNA、p-JAK2、p-STAT3蛋白表达降低,细胞凋亡率、IL-6、TNF-α水平、Bax蛋白表达升高(P<0.05)；AG490减弱了高剂量PTL对HG诱导的MIN6细胞损伤的改善作用。结论:PTL可能通过激活JAK/STAT信号通路促进HG诱导的MIN6细胞增殖,抑制细胞凋亡。 |
| 英文摘要: |
| Objective: To investigate the impact of parthenolide (PTL) on the injury of islet β cells induced by high glucose (HG) by regulating Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signal pathway. Methods: HG-induced mouse islet cell MIN6 cells were treated with 0, 2.5, 5, 10, 20, 40 μmol/L PTL, respectively, the appropriate PTL concentration for treating MIN6 cells was selected by detecting cell viability; MIN6 cells in logarithmic growth phase were separated into control group ( 5.5 mmol/L glucose), HG group (33.3 mmol/L glucose), HG+low-dose PTL group (33.3 mmol/L glucose+2.5 μmol/L PTL), HG+medium-dose PTL group (33.3 mmol/L glucose+5 μmol/L PTL), HG+high-dose PTL group (33.3 mmol/L glucose+10 μmol/L PTL), HG+AG490 (JAK/STAT pathway inhibitor) group (33.3 mmol/L glucose+40 μmol/L AG490), and HG+high-dose PTL+AG490 group (33.3 mmol/L glucose+10 μmol/L PTL+40 μmol/L AG490). The proliferation of MIN6 cells was detected by CCK-8; the apoptosis of MIN6 cells was detected by flow cytometry; the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and insulin content in cell supernatant were detected with the kit; the expression of proliferating cell nuclear antigen (PCNA), BCL2 associated X protein (Bax), p-JAK2, p-STAT3 protein in MIN6 cells was detected by western blot. Results: 2.5 μmol/L, 5 μmol/L and 10 μmol/L PTL were selected as the low, medium and high dose concentrations of HG-induced MIN6 cells. Compared with control group, the OD450 value, insulin content, PCNA, p-JAK2, p-STAT3 protein expression in HG group were lower, the apoptosis rate, IL-6, TNF-α levels and Bax protein expression were higher (P<0.05); compared with HG group, the OD450 value, insulin content, PCNA, p-JAK2, p-STAT3 protein expression in HG+low-dose PTL group, HG+medium-dose PTL group and HG+high-dose PTL group were higher, the apoptosis rate, IL-6, TNF-α levels and Bax protein expression were lower (P<0.05); compared with HG group, the OD450 value, insulin content, PCNA, p-JAK2, p-STAT3 protein expression in HG+AG490 group were lower, the apoptosis rate, IL-6, TNF-α levels and Bax protein expression were higher (P<0.05); AG490 attenuated the improvement effect of high dose PTL on HG-induced injury of MIN6 cells. Conclusion: PTL may promote HG-induced proliferation and inhibit apoptosis of MIN6 cells by activating JAK/STAT signal pathway. |
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