| 徐海飞,张伟,罗伟俊,雷建明.超声微泡协同骨髓间充质干细胞外泌体miR-98-5p对心肌梗死的治疗作用[J].浙江中西医结合杂志,2025,35(4): |
| 超声微泡协同骨髓间充质干细胞外泌体miR-98-5p对心肌梗死的治疗作用 |
| The therapeutic effect of ultrasound microbubbles combined with bone marrow mesenchymal stem cell exosome miR-98-5p on myocardial infarction |
| 投稿时间：2024-04-17 修订日期：2025-02-19 |
| DOI： |
| 中文关键词: 骨髓间充质干细胞外泌体 miR-98-5p 急性心肌梗死 超声微泡 |
| 英文关键词:bone marrow mesenchymal stem cell exosomes miR-98-5p acute myocardial infarction ultrasonic microbubbles |
| 基金项目:公益性技术应用研究项目 |
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| 中文摘要: |
| 目的 探究超声靶向微泡破坏（Ultrasound Targeted Microbubble Destruction，UTMD）协同骨髓间充质干细胞（Bone Marrow Mesenchymal Stem Cells，BMSC）外泌体miR-98-5p对心肌梗死的影响。方法 构建大鼠急性心肌梗死（Acute Myocardial Infarction，AMI）模型，UTMD递送BMSC外泌体miR-98-5p治疗AMI大鼠。SD大鼠随机分为AMI组、AMI+exosome组、AMI+mimic NC-exosome组、AMI+miR-98-5p mimic-exosome组、AMI+UTMD组、AMI+ miR-98-5p mimic-exosome+UTMD组，每组6只。实时荧光定量PCR检测miR-98-5p表达变化。TTC染色检测大鼠心脏梗死面积，共聚焦荧光显微镜检测DiR标记外泌体向心脏递送情况，TUNEL染色检测大鼠心脏组织细胞凋亡程度，ELISA检测大鼠血清心肌肌钙蛋白T（cTnT）、肌酸激酶同工酶（CK-MB）和乳酸脱氢酶（LDH）水平。结果 与Sham组相比，AMI大鼠中miR-98-5p低表达。BMSC外泌体miR-98-5p对大鼠AMI具有治疗作用，可减少心脏梗死面积，减弱细胞凋亡进程。UTMD能提高递送效率，从而更有效治疗大鼠AMI。结论 UTMD协同BMSC外泌体miR-98-5p能治疗AMI。 |
| 英文摘要: |
| Objective To investigate the effect of ultrasound targeted microbubble destruction ( UTMD ) combined with bone marrow mesenchymal stem cells ( BMSC ) exosome miR-98-5p on myocardial infarction. Methods The rat model of acute myocardial
infarction ( AMI ) was constructed, and UTMD was used to deliver BMSC exosome
miR-98-5p to treat AMI rats. SD rats were randomly divided into AMI group, AMI + exosome group, AMI + mimic NC-exosome group, AMI + miR-98-5p mimic-exosome group, AMI + UTMD group, AMI + miR-98-5p mimic-exosome + UTMD group, with 6 rats in each group. The expression of miR-98-5p was detected by real-time fluorescence quantitative PCR. TTC staining was used to detect the infarct size of rat heart. Confocal fluorescence microscopy was used to detect the delivery of DiR-labeled exosomes to the heart. TUNEL staining was used to detect the degree of apoptosis in rat heart tissue. ELISA was used to detect the levels of serum cardiac troponin T ( cTnT ), creatine kinase isoenzyme ( CK-MB ) and lactate dehydrogenase ( LDH ). Results Compared with the Sham group, the expression of miR-98-5p in AMI rats was lower. BMSC exosome miR-98-5p has a therapeutic effect on AMI in rats, which can reduce the area of cardiac infarction and weaken the process of apoptosis. UTMD can improve the delivery efficiency and thus more effectively treat AMI in rats. Conclusion UTMD combined with BMSC exosome miR-98-5 p can treat AMI. |
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