| 罗远明.骨折延迟愈合患者血清LINC01535和LINC00205水平的表达及临床意义[J].浙江中西医结合杂志,2025,35(7): |
| 骨折延迟愈合患者血清LINC01535和LINC00205水平的表达及临床意义 |
| Expression and clinical significance of serum LINC01535 and LINC00205 levels in patients with delayed fracture healing |
| 投稿时间：2024-08-06 修订日期：2024-12-20 |
| DOI： |
| 中文关键词: 骨折延迟愈合 长链非编码RNA01535 长链非编码RNA00205 临床意义 |
| 英文关键词:Delayed fracture healing Long-chain non-coding RNA01535 Long-chain non-coding RNA00205 Clinical significance |
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| 中文摘要: |
| 目的:探究骨折延迟愈合患者血清长链非编码RNA01535(LINC01535)和LINC00205水平的表达及临床意义。方法:选取2019年2月至2023年12月102例本院收治的骨折患者为观察组,根据患者愈合情况分为延迟组(46例)和正常组(56例),另选同期102例体检健康者为对照组。采用荧光定量PCR法测定血清中LINC01535、LINC00205的水平；多因素Logistic回归分析骨折延迟愈合的影响因素；ROC曲线分析血清LINC01535、LINC00205水平对骨折延迟愈合的预测价值。结果:与对照组相比,观察组血清LINC01535明显降低(P<0.05),LINC00205水平明显升高(P<0.05)；与正常组相比,延迟组患者的年龄、性别、BMI、骨折类型、骨折部位、饮酒史、吸烟史、高血压均无明显差异(P>0.05),骨质疏松、糖尿病的占比明显升高(P<0.05)；与正常组相比,延迟组血清LINC01535明显降低(P<0.05),LINC00205水平明显升高(P<0.05)；骨质疏松、糖尿病、LINC00205是骨折延迟愈合的独立危险因素(P<0.05),LINC01535是独立保护因素(P<0.05)；血清LINC01535、LINC00205水平预测骨折延迟愈合的曲线下面积(AUC)分别为0.824、0.834,二者联合预测的AUC为0.911,二者联合预测优于各指标单独预测(Z二者联合-LINC01535=1.959、Z二者联合-LINC00205=2.018,P=0.048、0.044),二者联合预测的敏感度为80.43%,特异性为92.86%。结论:骨折延迟愈合患者血清LINC01535水平下调,LINC00205水平上调,且LINC00205是骨折延迟愈合的独立危险因素,LINC01535是独立保护因素,二者联合对骨折延迟愈合具有较高的预测价值,可广泛应用于临床评估中。 |
| 英文摘要: |
| Objective: To investigate the expression and clinical significance of serum long chain non-coding RNA01535 (LINC01535) and LINC00205 levels in patients with delayed fracture healing. Methods: A total of 102 fracture patients admitted to our hospital from February 2019 to December 2023 were included as the observation group, which were separated into delayed group (46 cases) and normal group (56 cases) according to the healing status of the patients, and another 102 physically examined and healthy people in the same period were included as the control group. Fluorescence quantitative PCR was used to determine the levels of LINC01535 and LINC00205 in serum; multifactorial logistic regression was used to analyze the influencing factors of delayed fracture healing; and ROC curve was used to analyze the predictive value of serum levels of LINC01535 and LINC00205 for delayed fracture healing. Results: Compared with the control group, serum LINC01535 was greatly lower (P<0.05) and LINC00205 level was greatly higher (P<0.05) in the observation group. Compared with the normal group, there was no great difference in age, gender, BMI, fracture type, fracture site, history of alcohol consumption, history of smoking, and hypertension in the delayed group (P>0.05), and the percentages of osteoporosis and diabetes mellitus were greatly higher (P<0.05). Compared with the normal group, serum LINC01535 was greatly lower (P<0.05) and LINC00205 level was greatly higher (P<0.05) in the delayed group. Osteoporosis, diabetes mellitus, and LINC00205 were independent risk factors for delayed fracture healing (P<0.05), and LINC01535 was an independent protective factor (P<0.05). The area under the curve (AUC) of serum LINC01535 and LINC00205 levels for predicting delayed fracture healing was 0.824 and 0.834, respectively, and the AUC of the combination of the two was 0.911, and the combination of the two was superior to the individual prediction of each index (Z combination -LINC01535=1.959, Z combination -LINC00205= 2.018, P=0.048, 0.044), and the sensitivity of the combination was 80.43% and the specificity was 92.86%. Conclusion: Serum LINC01535 level is downregulated and LINC00205 level is upregulated in patients with delayed fracture healing. LINC00205 is an independent risk factor for delayed fracture healing, and LINC01535 is an independent protective factor. The combination of the two has a high predictive value for delayed fracture healing and can be widely used in clinical evaluation. |
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