| 高涵.血清HDAC3、Beclin-1、ACE表达与慢性牙周炎患者牙周病变程度及Th17/Treg失衡的关系[J].浙江中西医结合杂志,2025,35(10): |
| 血清HDAC3、Beclin-1、ACE表达与慢性牙周炎患者牙周病变程度及Th17/Treg失衡的关系 |
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| 投稿时间：2024-08-30 修订日期：2025-08-15 |
| DOI： |
| 中文关键词: 慢性牙周炎 组蛋白去乙酰化酶3 血管紧张素转换酶 Beclin-1 Th17/Treg 相关性 |
| 英文关键词:Chronic periodontitis Histone deacetylase 3 Angiotensin converting enzyme Beclin-1 Th17/Treg Correlation |
| 基金项目:浙江省医药卫生科技计划项目(2024KY1643) |
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| 中文摘要: |
| 目的:探讨血清组蛋白去乙酰化酶3(HDAC3)、Beclin-1、血管紧张素转换酶(ACE)表达与慢性牙周炎(CP)患者牙周病变程度及辅助性T细胞17(Th17)/调节性T细胞(Treg)失衡的关系。方法:选取2021年3月至2023年3月在本院口腔科门诊诊治的135例CP患者,作为研究组,根据探针深度(PD)及附着丧失(AL)的严重程度将其分为轻度组41例、中度组48例、重度组46例；另选取同期牙周健康人群135例作为对照组。酶联免疫吸附(ELISA)法检测HDAC3、Beclin-1、ACE水平；采用Pearson法分析CP患者血清HDAC3、Beclin-1、ACE与牙周相关指标及Th17/Treg的相关性；多因素Logistic回归分析CP的影响因素。结果:与对照组相比,研究组血清HDAC3、ACE水平均显著升高(P<0.05),Beclin-1水平显著降低(P<0.05)；轻度组、中度组和重度组患者血清HDAC3、ACE水平以及Th17/Treg值、SBI、PLI、PD、AL依次升高(P＜0.05),Beclin-1水平依次降低(P＜0.05)；根据Pearson相关性分析,血清HDAC、ACE与SBI、PLI、PD、AL以及Th17/Treg呈正相关(P＜0.05),血清Beclin-1与SBI、PLI、PD、AL以及Th17/Treg呈负相关(P＜0.05)；多因素Logistic回归分析得知,HDAC3、ACE为影响CP的危险因素(P＜0.05),Beclin-1为影响CP的保护因素(P＜0.05)。结论:CP患者血清HDAC3、ACE水平显著升高,Beclin-1水平显著降低,而且与疾病严重程度和Th17/Treg失衡有关。 |
| 英文摘要: |
| Objective: To explore the relationship between the expression of serum histone deacetylase 3 (HDAC3), Beclin-1, and angiotensin converting enzyme (ACE) with the degree of periodontal disease and the imbalance of helper T cell 17 (Th17)/regulatory T cell (Treg) in patients with chronic periodontitis (CP). Methods: From March 2021 to March 2023, 135 CP patients who were treated in the outpatient department of our hospital were regarded as the study group. They were separated into mild group (41 cases), moderate group (48 cases), and severe group (46 cases) based on severity of probe depth (PD) and attachment loss (AL); another 135 healthy individuals with periodontitis were as the reference group. Enzyme linked immunosorbent assay (ELISA) was performed to detect levels of HDAC3, Beclin-1, and ACE. Pearson method was performed to analyze the correlation between serum HDAC3, Beclin-1, ACE, periodontal related indicators, and Th17/Treg in CP patients. Multivariate logistic regression was performed to analyze the influencing factors of CP. Results: Compared with the reference group, the serum levels of HDAC3 and ACE in the study group were obviously increased (P<0.05), while the level of Beclin-1 was obviously reduced (P<0.05). The serum levels of HDAC3, ACE, Th17/Treg, SBI, PLI, PD, and AL in the mild, moderate, and severe groups increased sequentially (P<0.05), while the level of Beclin-1 decreased sequentially (P<0.05). According to Pearson correlation, serum HDAC and ACE were positively correlated with SBI, PLI, PD, AL, and Th17/Treg (P<0.05), while serum Beclin-1 was negatively correlated with SBI, PLI, PD, AL, and Th17/Treg (P<0.05). Multivariate logistic regression found that HDAC3 and ACE were risk factors affecting CP (P<0.05), while Beclin-1 was a protective factor affecting CP. Conclusion: The serum levels of HDAC3 and ACE in CP patients are obviously increased, while the level of Beclin-1 is obviously reduced, which is related to the severity of disease and Th17/Treg imbalance. |
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