| 赖庆钟,叶扬,刘敏,徐赟赟.蜂毒疗法对类风湿性关节炎大鼠炎症反应的作用研究[J].浙江中西医结合杂志,2025,35(6): |
| 蜂毒疗法对类风湿性关节炎大鼠炎症反应的作用研究 |
| Mechanistic Study of Bee venom therapy on Inflammatory Response in Rats with Rheumatoid Arthritis |
| 投稿时间：2024-09-16 修订日期：2025-03-05 |
| DOI： |
| 中文关键词: 大鼠 类风湿关节炎 蜂毒疗法 肿瘤坏死因子-α 白细胞介素-1β |
| 英文关键词:Rheumatoid Arthritis Bee venom therapy Adjuvant Arthritis Inflammatory Response |
| 基金项目: |
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| 中文摘要: |
| 摘要：目的 探讨蜂毒疗法对类风湿性关节炎(RA)模型大鼠炎症反应的治疗作用。方法 24只SPF级雄性SD大鼠按照随机数字表法分为正常组、模型组和蜂疗组，每组8只。除正常组外，模型组及蜂疗组均采用左后足跖皮内注射弗氏完全佐剂建立RA模型。造模完成后，蜂疗组每周活蜂蜇刺1次，持续6周，正常组和模型组予以同体积生理盐水皮下注射，并记录各组大鼠的一般情况和体质量变化。实验结束后使用数字游标卡尺测量及记录大鼠足跖肿胀度相关数值；采用酶联免疫吸附测定(ELISA)检测大鼠血清肿瘤坏死因子(TNF)-α和白细胞介素(IL)-1β的含量变化；通过X射线及苏木精-伊红(HE)染色法观察大鼠踝关节病理变化。结果 与模型组比较，蜂疗组大鼠精神状态、活动量和毛发状况有所改善，左足跖肿胀显著降低[(7.74±0.4)mm比(8.44±0.42) mm, P<0.05]。与正常组比较，模型组大鼠血清IL-1β[(10.35±0.23)pg/mL比(5.86±0.25) pg/mL, P<0.01]及TNF-α[(43.14±4.53)pg/mL比(22.47±3.93) pg/mL, P<0.05]含量显著升高，而蜂疗组大鼠血清IL-1β[(9.84±0.29)pg/mL比(10.35±0.23) pg/mL, P<0.05]和TNF-α[(35.13±2.69)pg/mL比(43.14±4.53) pg/mL, P<0.05]含量显著降低。X射线及HE染色观察显示，模型组踝关节滑膜结构破坏及炎性细胞浸润，蜂疗组大鼠踝关节组织病变有一定程度的改善。结论 蜂毒疗法可明显缓解RA模型大鼠关节肿胀程度，缓解关节病理性破坏，其作用机制可能与抑制炎症细胞因子IL-1β、TNF-α的产生有关。 |
| 英文摘要: |
| Abstract Objective To investigate the therapeutic effect of bee venom therapy on inflammatory response in rats with rheumatoid arthritis ( RA ). Methods Twenty-four
SPF male SD rats were randomly divided into a normal group, a model group, and a bee venom therapy group according to the random number table method, with 8 rats in each group. Except for the normal group, the model and bee venom therapy groups were treated with intraplantar injection of Freund's complete adjuvant into the left hind foot to establish the RA model. After modeling, bee stings were performed in the bee venom therapy group once a week for 6 weeks, while the normal and model groups were treated with an equal volume of normal saline via subcutaneous injection. Then, the general condition and body weight of rats in each group were recorded. After the experiment, the digital vernier caliper was used to measure the swelling degree of the rat foot ; enzyme-linked immunosorbent assay ( ELISA ) was used to detect the changes of tumor necrosis factor ( TNF ) -α and interleukin ( IL ) -1β in serum of rats. X-ray and hematoxylin-eosin staining ( HE staining ) were used to observe the pathological changes of ankle joint in rats. Results Compared with the normal group, the mental state, activity and hair status of the rats in the bee venom therapy intervention group were improved. Compared with the model group, the left foot swelling of the bee venom therapy intervention group was significantly reduced [(7.74±0.4) vs. (8.44±0.42) mm, P<0.05]. Compared with the normal group, the contents of IL-1β[(10.35±0.23) vs. (5.86±0.25) pg/mL, P<0.01] and TNF-α[(43.14±4.53) vs. (22.47±3.93) pg/mL, P<0.05] in the serum of the model rats were significantly increased, while the contents of IL-1β[(9.84±0.29) vs. (10.35±0.23) pg/mL, P<0.05] and TNF-α[(35.13±2.69) vs. (43.14±4.53) pg/mL, P<0.05] were significantly decreased after bee venom therapy. X-ray and HE staining showed that the synovial structure of the ankle joint in the model group was destroyed and inflammatory cell infiltration was observed. The lesions of the ankle joint tissue in the bee venom therapy intervention group were improved to a certain extent. Conclusion Bee venom therapy can significantly alleviate the degree of joint swelling in RA rats and alleviate the pathological damage of joints. The mechanism may be related to the inhibition of the production of inflammatory cytokines IL-1β and TNF-α. |
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