| 周海丽,刘喜德.温化蠲痹方通过抑制自噬调节巨噬细胞极化治疗类风湿关节炎[J].浙江中西医结合杂志,2025,35(2): |
| 温化蠲痹方通过抑制自噬调节巨噬细胞极化治疗类风湿关节炎 |
| Wenhua Juanbi Recipe regulates macrophage polarisation by inhibiting autophagy in the treatment of rheumatoid arthritis |
| 投稿时间：2024-11-01 修订日期：2025-01-08 |
| DOI： |
| 中文关键词: 类风湿关节炎 温化蠲痹方 自噬 巨噬细胞极化 |
| 英文关键词:Rheumatoid Arthritis Wenhua Juanbi Recipe Autophagy Macrophage polarisation |
| 基金项目:杭州市卫生科技计划重大项目(Z20200026) |
|
| 摘要点击次数: 252 |
| 全文下载次数: 2 |
| 中文摘要: |
| 目的 研究温化蠲痹方对胶原诱导性关节炎（CIA）大鼠自噬和巨噬细胞极化的影响。方法 将50 只Wister雌性大鼠随机分为正常组（n=10 只）和造模组（n=40只）。造模组采用大鼠尾根部注射BCII乳剂，建立CIA大鼠模型。选取30 只造模成功的大鼠随机分为模型组、MTX组、WJR组，正常组和模型组给予2mL 0.9 %氯化钠溶液，每天1次，MTX组给予MTX混悬液0.78 mg/kg，每周1次，WJR组给予WJR 22.9 g/kg，每天1次。灌胃持续4 周。观察各组大鼠的一般情况，记录关节炎指数评分，免疫荧光检测正常组和模型组大鼠滑膜组织中LC3II的平均荧光强度，Western-blot检测大鼠滑膜组织中Beclin1、LC3Ⅱ、CD16、CD206蛋白表达，RT-qPCR检测大鼠滑膜组织中ATG5、ATG7、ATG12 基因表达，酶联免疫吸附试验检测大鼠滑膜组织中IL-6、TNF-α、IL-10及Arginase1水平。结果 与正常组比较，模型组大鼠关节明显肿胀，关节炎指数评分升高，与模型组比较，治疗后MTX组、WJR组大鼠关节炎指数评分[（2.70±0.30）分、（2.40±0.16）分比（8.20±0.59）分，P＜0.05]降低，与本组治疗前相比，治疗后MTX组、WJR组大鼠关节炎指数评分降低。与正常组比较，模型组LC3Ⅱ的平均荧光强度增加，Beclin1、LC3Ⅱ、CD16蛋白表达增加，CD206蛋白表达降低，ATG5、ATG7、ATG12 基因表达升高，IL-6、TNF-α表达升高，IL-10、Arginase1表达降低。与模型组比较，MTX组、WJR组ATG5[（20.40±1.07）、（6.46±0.77）比（71.07±2.73），P＜0.05]、ATG7[（52.41±1.50）、（17.82±1.63）比（148.41±2.30），P＜0.05]、ATG12[（35.58±1.47）、（23.01±0.62）比（59.43±0.60），P＜0.05] 基因表达降低，Beclin1[（0.35±0.01）、（0.27±0.02）比（0.82±0.02），P＜0.05]、LC3Ⅱ[（0.47±0.01）、（0.14±0.01）比（0.57±0.02），P＜0.05]、CD16[（0.45±0.01）、（0.46±0.01）比（0.88±0.01），P＜0.05]蛋白表达降低，WJR组CD206[（0.63±0.03）比（0.52±0.01），P＜0.05]表达升高，MTX组CD206[（0.32±0.01）比（0.52±0.01），P＜0.05]表达降低，MTX组、WJR组IL-6[（37.27±1.22）pg/mL、（18.81±1.03）pg/mL比（109.23±0.85）pg/mL，P＜0.05]、TNF-α[（22.58±0.52）pg/mL、（9.75±0.54）pg/mL比（431.22±6.79）pg/mL，P＜0.05]表达降低，IL-10[（43.23±0.59）pg/mL、（67.08±2.11）pg/mL比（9.33±0.47）pg/mL，P＜0.05]、Arginase1[（65.72±0.50）pg/mL、（91.01±0.92）pg/mL比（22.17±0.58）pg/mL，P＜0.05]表达升高。结论 温化蠲痹方可能通过抑制自噬调节巨噬细胞极化治疗类风湿关节炎。 |
| 英文摘要: |
| Objective To study the effect of Wenhua Juanbi Recipe(WJR) on autophagy and macrophage polarisation in rats with collagen-induced arthritis (CIA). Methods Fifty female Wistar rats were randomly divided into two groups：a normal group（n=10）and a model group（n=40）. CIA model was established by intracaudal multi-point injection of BCII emulsifier. Thirty successfully modeled CIA rats were randomly divided into a model group（n=10）,a WJR group（n=10）and a MTX group（n=10）. The normal group and the model group were given 2 mL 0.9 % sodium chloride solution, once a day. The MTX group was given MTX suspension 0.78 mg / kg, once a week, and the WJR group was given WJR 22.9 g / kg, once a day. Gavage was continued for 4 weeks. Observe the general conditions of the rats in each group and record athritis index scores. Mean fluorescence intensity of microtubule-associated protein LC3II in synovial tissue of rats in normal and model groups detected by immunofluorescence. Western blot was used to detect the expression of Beclin1, LC3II, CD16 and CD206, as well as real-time quantitative PCR for the expression of ATG5, ATG7, and ATG12 . Expression of IL-6, TNF-α, IL-10 and Arginase1 cytokines was determined by ELISA. Results Compared to the normal group, rats in the model group had significantly swollen joints and elevated athritis index scores. Compared to the model group, the athritis index scores of rats in the MTX and WJR groups [(2.70±0.30)points, (2.40±0.16)points vs. (8.20±0.59)points, P<0.05] decreased after treatment. Compared to the pre-treatment of this group, the athritis index scores of rats in MTX and WJR groups were reduced after treatment. Compared to the normal group, the mean fluorescence intensity of LC3II in the model group increased, Beclin1, LC3II, CD16 were increased, CD206 was decreased, ATG5 , ATG7, ATG12 were elevated, IL-6, TNF-αexpression were elevated, and IL-10 , Arginase1 expression were decreased. Compared to the model group, ATG5 [(20.40±1.07), (6.46±0.77) vs. (71.07±2.73), P<0.05], ATG7 [(52.41±1.50), (17.82±1.63) vs. (148.41±2.30), P<0.05], ATG12 [( 35.58±1.47), (23.01±0.62) vs. (59.43±0.60), P<0.05] were reduced, and Beclin1 [(0.35±0.01), (0.27±0.02) vs. (0.82±0.02), P<0.05], LC3II [(0.47±0.01), (0.14± 0.01) vs. (0.57 ± 0.02), P < 0.05], CD16 [(0.45 ± 0.01), (0.46 ± 0.01) vs. (0.88 ± 0.01), P < 0.05] were decreased in MTX and WJR groups, CD206 expression [(0.63 ± 0.03) vs. (0.52 ± 0.01), P < 0.05] was elevated in WJR group, and expression [(0.32±0.01) vs. (0.52±0.01) was decreased in MTX group, P<0.05], and the MTX and WJR groups showed IL-6 [(37.27±1.22)pg/mL, (18.81±1.03)pg/mL vs. (109.23±0.85)pg/mL, P<0.05], TNF-α[(22.58±0.52)pg/mL, (9.75±0.54) pg/mL vs. (431.22±6.79)pg/mL, P<0.05] were decreased, IL- 10 [(43.23±0.59)pg/mL, (67.08±2.11)pg/mL vs. (9.33±0.47)pg/mL, P<0.05], Arginase1 [(65.72±0.50)pg/mL, (91.01±0.92) pg/mL vs. (22.17±0.58)pg/mL, P<0.05] were elevated. Conclusion WJR may regulate macrophage polarisation by inhibiting autophagy in the treatment of rheumatoid arthritis. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
