| 谭晓婵,贺晓旭,徐福.基于生物信息学、网络拓扑策略探究针刺治疗卒中后认知障碍的作用机制[J].浙江中西医结合杂志,2025,35(8): |
| 基于生物信息学、网络拓扑策略探究针刺治疗卒中后认知障碍的作用机制 |
| Exploring the mechanism of acupuncture for Post Stroke Cognitive Impairment based on bioinformatics and network topology strategies |
| 投稿时间：2025-02-03 修订日期：2025-05-12 |
| DOI： |
| 中文关键词: 针刺疗法 卒中后认知障碍 生物信息学 信号通路 分子机制 |
| 英文关键词:Acupuncture therapy Post Stroke Cognitive Impairment Bioinformatics Signaling pathway Molecular mechanis |
| 基金项目: |
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| 中文摘要: |
| 目的:本研究旨在运用文本挖掘、生物信息学及网络拓扑学等技术,探讨针刺对卒中后认知障碍(PSCI)的多靶点治疗机制。方法:通过文献检索获取针刺后产生的活性化合物,使用STITCH和SwissTargetPrediction数据库预测其潜在作用靶点。利用在线人类孟德尔遗传数据库(OMIM)、GeneCards基因数据库和UniProt蛋白资源数据库筛选出与PSCI有关的疾病基因靶点。使用Cytoscape3.10.1软件构建共有靶点的蛋白质-蛋白质互作网络,并借助DAVID数据库对这些靶点进行基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)富集分析,同时进行可视化处理。结果:鉴定出249个与针刺治疗PSCI相关的基因靶点,并筛选出146条通路。结论:研究预测,针刺可能通过AKT1、TP53、EGFR、CASP3、mTOR等关键靶点,调节MAPK信号通路、P53信号通路和HIF-1信号通路,进而调节脑内多巴胺及5-羟色胺等神经递质的水平,从而改善神经元功能；此外,针刺可能抑制炎症反应,减少氧化应激,以保护神经元免受损伤,促进神经干细胞增殖和分化,增强神经血管生成,改善脑血液循环,促进神经再生。 |
| 英文摘要: |
| Objective: To investigate the multi-target mechanism of acupuncture for Post Stroke Cognitive Impairment (PSCI) using text mining, bioinformatics, and network topology. Methods: Literature was searched to discover active compounds produced after acupuncture, and potential targets of action were predicted by STITCH and SwissTargetPrediction databases. PSCI-associated disease gene targets were screened using the Human Gene Database, the Online Human Mendelian Inheritance Database, and the Global Protein Resources Database. Cytoscape 3.10.1 software was used to construct protein-protein interaction network maps of shared targets, and gene ontology enrichment analysis and Kyoto Genome Encyclopedia enrichment analysis of shared targets were performed and visualized by DAVID database, respectively. Results: A total of 249 gene targets related to acupuncture for Parkinson"s disease were identified, and 146 pathways were screened. Conclusion: This study predicts that acupuncture may regulate the MAPK signaling pathway, P53 signaling pathway, and HIF-1 signaling pathway through key targets such as AKT1, TP53, EGFR, CASP3, and mTOR, regulate the levels of neurotransmitters such as dopamine and 5-hydroxytryptophan in the brain, and improve the function of neurons; inhibit inflammatory responses, reduce oxidative stress, protect neurons from damage, and promote the neural stem cells proliferation and differentiation, promote neuroangiogenesis, improve cerebral blood circulation, and promote nerve regeneration. |
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