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| 葛花提取物葛花苷通过PI3K-Akt信号通路抑制血小板活化 |
| Pueraria lobata extract kakkalide inhibits platelet activation via PI3K-Akt signaling pathway |
| 投稿时间：2025-06-22 修订日期：2026-04-12 |
| DOI： |
| 中文关键词: 葛花苷 葛花 抗血小板治疗 PI3K Akt |
| 英文关键词:Kakkalide Pueraria lobata Antiplatelet therapy PI3K Akt |
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| 中文摘要: |
| 【目的】:探索葛花苷对血小板活化的影响及其潜在机制。【方法】:通过血小板聚集、释放、铺展、栓快回缩等功能试验研究葛花苷对人血小板活化的影响,采用蛋白质印迹法检测葛花苷对thrombin诱导的血小板Akt磷酸化的影响,通过血小板聚集实验观察PI3K抑制剂LY294002、Akt抑制剂Akt-IN-6是否拮抗葛花苷的抗血小板作用。【结果】:葛花苷浓度依赖的抑制了thrombin、collagen、ADP诱导的人血小板聚集、释放,葛花苷抑制了血小板铺展、栓快回缩,葛花苷浓度依赖的抑制了thrombin诱导的血小板Akt磷酸化,葛花苷的抗血小板作用在PI3K抑制剂LY294002、Akt抑制剂Akt-IN-6处理的血小板中消失。【结论】:葛花提取物葛花苷通过PI3K-Akt信号通路抑制血小板活化。 |
| 英文摘要: |
| [Objective] To explore the effects of Kakkalide on platelet activation and its underlying mechanisms. [Methods] The effects of Kakkalide on human platelet aggregation, secretion, spreading, and clot retraction were investigated. Western blotting was used to detect the effect of Kakkalide on thrombin-induced platelet Akt phosphorylation. Platelet aggregation experiments were performed to observe whether the PI3K inhibitor LY294002 and the Akt inhibitor Akt-IN-6 could antagonize the anti-platelet effects of Kakkalide. [Results] Kakkalide inhibited thrombin-, collagen-, and ADP-induced human platelet aggregation and secretion in a concentration-dependent manner. Kakkalide also inhibited platelet spreading and clot retraction. Kakkalide inhibited thrombin-induced platelet Akt phosphorylation in a concentration-dependent manner. The anti-platelet effects of Kakkalide were abolished in platelets treated with the PI3K inhibitor LY294002 and the Akt inhibitor Akt-IN-6. [Conclusion] Pueraria lobata extract Kakkalide inhibits platelet activation via PI3K-Akt signaling pathway. |
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