| Objective To study the variety and differentiation of intestinal flora in Triple Negative Breast Cancer(TNBC) patients with diverse traditional Chinese medicine syndromes.Methods Twenty-one patients with TNBC who met the criteria were included as the patient group,including 5 patients with spleen-stomach deficiency syndrome,8 patients with liver and kidney yin deficiency syndrome,and 8 patients with liver-qi stagnation syndrome.Meanwhile,10 healthy persons were included as the control group. The stooll samples were collected and the richness and distribution of intestinal flora were tested by 16S rDNA sequencing technology.The differences between groups were analyzed to find bacteria with significant differences between different groups(P<0.05).Results There was no significant difference in bacterial diversity between TNBC patients and normal group, and between different TNBC syndrome types (P>0.05).The content of flora in patients with TNBC was significantly less than that in the normal group (P<0.05), including Lachnospira, Prevotellaceae, Mitsuokella, Coprococcus, Odoribacter, Enterococcus and so on. Compared with the healthy control group, there were significant enrichment in the spleen-stomach deficiency syndrome group including Prevotella, Lactobacillus, Tyzzerella, Dyadobacter, Deinococcus, Actinobacillus and other bacteria (P<0.05).The bacteria with significant enrichment (P<0.05) in the liver-kidney yin deficiency syndrome group were Tyzzerella, Gordonibacter, Hungatella and other bacteria (P<0.05).In the group of liver stagnation and qi stagnation, Flavonifractor, Proteus, Enhydrobacter, Citrobacter, Anaeroglobus, Acetobacter and so on were significantly enriched(P<0.05).Conclusion The constructions of intestinal flora between patients with TNBC and healthy people are markedly different, and the constructions of intestinal flora in patients with different syndromes of TNBC are also ovbiously disparate.The occurrence of TNBC is related to the increase of pernicious flora like Hungatella and the decrease of beneficial flora like Coprococcus. There is also a certain relationship between the syndrome differences of various TCM syndromes and the changes of bacteria in the intestinal environment. |
